In short:

Australians Alex de Minaur and Rinky Hijikata have won their round of 64 matches at the Cincinnati Open.

Novak Djokovic was beaten in three sets by Argentine Thiago Agustin Tirante.

What's next?

De Minaur will next face Brit Arthur Frey, while Hijikata meets Jakub Menšík from Czechia.

The Australian men's assault at the Cincinnati Open has continued with gusto thanks to Alex de Minaur and Rinky Hijikata.

A day after James Duckworth, Adam Walton and Chris O'Connell scored first-round wins, but Thanasi Kokkinakis was beaten, de Minaur was pushed to his limits at the final Masters 1000 event before the US Open at Flushing Meadows.

Having dropped the first set in just 26 minutes, fifth seed de Minaur, who received an opening-round bye, responded to defeat Quentin Halys 1-6, 6-3, 7-6 (7-4).

The Frenchman had won their only previous meeting, a Miami Masters hard-court clash, back in 2023.

De Minaur lost his opening two service games of the match, but was not broken again en route to setting up a round-of-32 clash with Brit Arthur Fery, who saw off Duckworth, 6-4, 2-6, 7-6, (7-4) in a tick over two-and-a-half hours.

It will be the first clash on tour between de Minaur and the 32nd-seeded French-born Fery.

Earlier on Saturday (Sunday AEST), 25-year-old wildcard Hijikata beat 19th seed Luciano Darderi, of Italy, 6-4, 6-2 to set up his last-32 match with 14th seed Jakub Mensik.

Novak Djokovic was beaten in three sets, by Argentine Thiago Agustin Tirante.

Miomir Kecmanovic, Hubert Hurkacz and Mattia Bellucci were also defeated.

Playing his first match ‌since losing a Wimbledon semifinal to Jannik Sinner, 39-year-old Djokovic struggled in the heat and required a ‌medical timeout in a disappointing US Open tune-up.

"Congrats to my opponent," Djokovic said after losing to the world number 50.

"It was not an enjoyable match for me, for sure. The way I felt. Not the first and I guess not the last time, but it is what it is."

The Serbian — a three-time Cincinnati Open champion and number five in the rankings — will look to regroup before heading to New York, where he ⁠will seek a record-extending 25th grand slam singles ​title.

"I think this is the best win of my career," Tirante said.

"I think I really did a good job inside the court. I managed very well the nerves of playing against a legend like Novak."

ABC/wires